جامعة بلاد الرافدين - Bilad Alrafidain University - د. حامد خليل علي التميمي

د. حامد خليل علي التميمي

Dr.Hamid Khaleel Ali AL-Tameemi

مقرر قسم : قسم تقنيات المختبرات الطبية

Department rapporteur : DEPARTMENT OF MEDICAL LABORATORY TECHNIQUES

دكتوراه امراض نسيجية

Ph.D Histopatholgy

dr.hamed@bauc14.edu.iq

hamid.altameme@yahoo.com

السيرة الذاتية

نشاطات التدريسي

البحوث المنشورة

المؤلفات

المقررات المكلف بها

المحاضرات

مشاريع التخرج

المقالات داخل المجلة

المقررات المكلف بها

المقررات المكلف بها - 1

القسم	المرحلة	الفصل	رمز المقرر	الوحدات	توصيف	المقرر
قسم تقنيات المختبرات الطبية	المرحلة الثالثة	فصل اول		6		الامراض النسيجية 1

المحاضرات الالكترونية

المحاضرات الالكترونية - 6

العام	المقرر	القسم	المرحلة	المحاضرة
2024-2025	الامراض النسيجية 1	قسم تقنيات المختبرات الطبية	المرحلة الثالثة	Cellular adaptations
2024-2025	الامراض النسيجية 1	قسم تقنيات المختبرات الطبية	المرحلة الثالثة	healing and regeneration
2024-2025	الامراض النسيجية 1	قسم تقنيات المختبرات الطبية	المرحلة الثالثة	chronic inflammation
2024-2025	الامراض النسيجية 1	قسم تقنيات المختبرات الطبية	المرحلة الثالثة	acute inflammation
2024-2025	الامراض النسيجية 1	قسم تقنيات المختبرات الطبية	المرحلة الثالثة	Cell and its organelles
2024-2025	الامراض النسيجية 1	قسم تقنيات المختبرات الطبية	المرحلة الثالثة	Apoptosis

البحوث

	2023	AIP
Colorectal cancer is one of most common cancers that cause death, it consider third cause for death. Among the gastrointestinal tract cancers, it is more common in Europe and United States of America than Affrica and Asia. The aim of current study to investigate the association between any molecular aberration in Tp53 gene, exon 5 and mutant p53 protein expression in colorectal carcinoma development. Methods. The current study had two lines: molecular studies in the biology department of the Faculty of Sciences / University of Kufa, and immunohistochemical studies in the histopathology department of the Faculty of Medicine / University of Kufa. The current study was conducted between December 2019 and March 2021. The Scientific Ethical Committee's consents were obtained from all participants' prior to the collection of samples.

	2023	AIP
Colorectal carcinoma one of most incidence and dangerous cancer around the world. The aim of current study to investigate the association between any molecular aberration in PIK3CA gene, exon 20 and PIK protein expression in colorectal carcinoma development. Methods; The current study achieved in two lines, Molecular study which achieved in advanced researches laboratory in biology department, Faculty of Sciences / University of Kufa, while immunohistochemical study was achieved in histopathology department, faculty of Medicine / University of Kufa. Only Iraqi patients were included in the current study who diagnosed through period extend from 01 December 2019 to 30 February 2021 and any patients diagnosed before or after this period were excluded. Also any samples without case report were excluded. The present study included 103 cases divided into two groups, patients group were included 50 cases with colorectal carcinoma(adenocarcinoma) and 53 cases with other non-adenocarcinoma such as hyperplastic polyp and colitis. According to gender, a study was included 61 male and 42 female. According to patients ages. the average was(50-89) years. The samples were collected from AL-Sader medical city in AL-Najaf province and Medical city in Baghdad province. Blood samples and tissues samples were collected from each 103 patients after enter the hospital(s) and have report of colonoscopy or CT-scan. blood samples were used in molecular line to (DNA extraction and sequencing). Tissues sample were confirmed by H&E examination and from these 102 cases only 50 cases with and other cases were excluded from a study. Breast carcinoma used as positive control for PIK3CA. Results: 27 cases (54%) given positive for staining with PIK3CA antibodies, but with normal genotype in sequencing. Therefore, there was no any correlation between colorectal carcinoma development and any aberration in PIK3CA gene –Exon 20 in current study. According to relation between colorectal carcinoma grades and positive and negative for PIK3CA, 15 cases(44%) in grade I, 8 cases (67%) in grade II and 4 cases (100%) were given positive for PIK3CA. There is positive correlation between grades and both ages and positive and negative cases( P =0.173 and sig=0.229) (P 0.312, sig 0.027) respectively. while, Negative correlation found between gender with both age and grades (p= -0.304, sig= 0.032) (P -0.365, sig=0.009) respectively. Also the negative correlation found between positive or negative cases for PIK3CA with both age and gender (P -0.196, sig 0.172)(P -0.201, sig 0.162) respectively. Conclusion: Due to relation between molecular study of PIK3CA Exon 20 and IHC study of PIK3CA expression, we don't found any relation between PIK3CA expression in patients with colorectal carcinoma and genetic aberration in PIK3CA gene, exon 20. According to relation between PIK3CA score index and grades, increase in PIK3CA score index directly Proportional with advance in grade of colorectal carcinoma. According to grades and cases with positive or negative cases, found numbers of cases that give positive for PIK3CA Abs increase with advance in grades.., There is positive correlation between grades and both ages and positive and negative cases for PIK3CA. while, Negative correlation found between gender with both age and grades. Also the negative correlation found between positive or negative cases for PIK3CA with both age and gender.

	2022	Open Access Macedonian Journal of Medical Sciences
Honey one of honeybee product that consider main food in many meals and have vital role in treatment a lot of medical problems. However, can be consider source of problems to human being around the world. Although the case of honey anaphylaxis very rare but so dangerous and may be life-threatening. In Iraq, I investigated and documented one case with honey allergy by skin prick-prick test. A patient with honey allergy has symptoms from mild to severe complications. Medical centres have no any background about this type of allergy, therefore it important to explain this case to prevent mis-diagnosis.

	2017	Journal of Pharmaceutical Sciences and Research Preview publication details ;
Objective: the objectives of the study were to comparative measurement of CEA and TAG 72 in sera and tissue of patients with colorectal tumors and determining whether serum biomarkers are diagnostic markers or not. Methods: 105 cases were included in this study divided into 3 groups of patients, patients with colorectal carcinoma, patients with adenomatous polyps(adenoma) and patients with non-specific colitis, 57 male and 48 female, 20 male and 15 female of carcinoma cases. Age range 42-79 years with mean 60.5 years. Paraffin blocks and blood samples were collected from these three group. Results: TAG 72 was shown more specificity and sensitivity than CEA marker in diagnosis colorectal carcinoma and adenomatous polyps, their concentration in colorectal carcinoma more than adenomatous polyps. CEA and TAG 72 were given positive as percentages for poorly differentiation carcinoma more than moderated and well differentiated. Conclusion: Biomarkers may aid in differentiating benign from malignant carcinoma. Measurement of the level of biomarkers in the serum is better than the tissue in the detection and differentiation between types of tumors. Serum biomarkers are reliable markers in detecting colorectal carcinoma. Complementary use of CEA and TAG 72 give more accurate results.

	2014	Heliyon
"Background Colorectal cancer ranks second as a cause of cancer deaths. Mutations in the adenomatous polyposis coli (APC) gene, especially in exon 16, could contribute to colorectal carcinoma development. This study explored the correlations between APC gene exon 16 variations/expression and colorectal carcinoma progression. Methods In a case-control study, blood samples from 150 colorectal carcinoma patients and 50 healthy volunteers were analyzed by PCR and sequencing for APC exon 16 variations. The APC protein expression on tissue samples was evaluated by immunohistochemistry and statistical analyses were used to examine clinicopathological correlations. Results The sequencing analysis revealed a mutation in exon 16 of the APC gene (rs459552) in 36 % of colorectal cancer cases while absent in all non-cancer controls. Subgroup analysis by tumor grade showed higher prevalence of mutant allele in Grade II and Grade III cases, with frequencies reaching 60.0 % and 69.2 %, respectively, compared to a substantially lower prevalence of 29.4 % in Grade I patients. Immunohistochemistry showed no significant correlation between this mutation and APC expression. APC positivity proportions were 25.5 % in Grade I tumors (n = 26/102) versus 17.1 % in Grade II (n = 6/35) and 46.2 % in Grade III (n = 6/13), showing a non-significant trend of reduced positivity in higher grade tumors (p>0.05). Conclusions The frequency of APC exon 16 mutation (rs459552) rose significantly with increasing tumor grade. Similarly, although not statistically significant, the percentage of APC positive staining increased with poorer tumor differentiation, rather than declining. Therefore, the APC exon 16 mutation and expression analysis provides insights into colorectal cancer progression, with the rs459552 mutation correlating with grade and may promoting aggression."

	2024	Malaysian Journal of Microbiology
"Aims: This study aimed to determine the level of CD17 and TLR-4 in the serum of patients with bacterial urinary tract infections (UTI). Methodology and results: 350 blood and 350 urine samples were collected from people with UTIs. After urine diagnosis, it was found that 205 patients had UTI infections. The results indicate that Gram-negative bacteria constituted 58.5% of the 123 isolates. They included Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and B. cepacia. At the same time, Gram-positive bacteria constituted 41.4% of total isolates, divided into two groups that include 37 isolates (30.08%) were Staphylococcus spp. and 14 isolates (11.3%) identified as Enterococcus spp. Staphylococcus spp. included as S. aureus and coagulase-negative Staphylococcus strains, identified as S. haemolyticus, S. epidermidis, S. vitulinus, S. sciuri, S. homins, S. warneri, S. lentus and Enterococcus spp. isolates that included E. faecalis, E. faecium and E. avium. The study found that the incidence of UTI was high among females 57.7% and males 42.3%. Immunological parameters included the determination of serum CD17 and TLR-4 levels. Conclusion, significance and impact of study: Results show that high level of CD17 and TLR-4 was found in UTI patients compared with the control group as 1.848 ± 0.588 versus 0.708 ± 0.229 µg/L and 841.0285 ± 67.7 versus 571.716 ± 21.8 pg/mL, respectively. "

	2024	Biomedical Research and Therapy
"Abstract Sepsis remains a critical health concern with high mortality despite medical advances. Early diagnosis and risk stratification are vital to guide appropriate intervention and improve outcomes. Plasma gelsolin, an actin-binding protein that helps clear circulating filamentous actin, has emerged as a promising diagnostic and prognostic biomarker for sepsis. This review summarizes evidence on the usefulness of plasma gelsolin for these applications. Studies consistently show significantly decreased plasma gelsolin levels in septic humans and animal models compared to healthy controls. Reductions correlate with sepsis severity and development of multiorgan dysfunction. Plasma gelsolin demonstrates a potential to distinguish sepsis from non-infectious inflammation. It also aids in mortality prediction, with lower levels portending worse outcomes. One study found that sepsis non-survivors failed to recover depleted gelsolin levels over time, in contrast to survivors. The degree of gelsolin depletion is likely connected to the extent of cellular injury from sepsis, with consumption overwhelming plasma gelsolin’s actin-scavenging capacity. Resulting persistent circulation of actin filaments is posited to mediate organ damage. Though most studies are limited by small sample sizes, plasma gelsolin consistently correlates with clinical deterioration. Substantiating its prognostic utility could enable risk stratification to guide sepsis management. Demonstrating a mortality benefit of gelsolin replacement therapy could also spur the development of novel treatments. Further research on plasma gelsolin is warranted to ultimately improve outcomes of this common and deadly syndrome."

	2022	Iranian Journal of War and Public Health
"Aims This study aimed to detect gram-positive bacteria that cause UTIs with multiple antibiotic resistance, as well as phenotype and genotype methods to determine some genes in S. aureus isolates. Materials & Methods This study included 205 urine samples from outpatients with a urinary tract infection. The Polymerase Chain Reaction method was performed to evaluate the genotypic status of the 16S rRNA, erm B, mecA, bla Z, and msrA genes after DNA extraction. Findings Only 51 (41.4%) were found to have gram-positive bacteria, isolated bacteria were divided into two groups that include 37 isolates (30.08%) were Staphylococcus spp. and 14 isolates (11.3%) diagnosed as Enterococcus spp. and Staphylococcus spp. divided into two groups that include 17 isolates (13.8%) diagnosed as S. aureus and 20 isolates (16.2%) diagnosed as coagulase-negative staphylococci, distributed as 7 isolates (5.6%) diagnosed as S. haemolyticus, 3 isolates (3.4%) diagnosed as S. epidermidis, 1 isolate (0.8%) diagnosed as S. vitulinus,4 isolates (3.2%) diagnosed as S. sciuri, 3 isolates (2.4%) diagnosed as S. homins, 1 isolate (0.8%) diagnosed as S. warneri, 1 isolate (0.8%) diagnosed as S. lentus. Conclusion The most common gram-positive bacteria found to cause UTIs was S. aureus. A few pathogens of S. aureus were discovered to be resistant to Vancomycin and linezolid in this study. The prevalence of blaZ genes, which are responsible for resistance, was found to be high among pathogenic S. aureus isolates in Diyala province."

	2024	Osol Journal of Medical Sciences
Rheumatoid arthritis [RA] is a persistent inflammatory polyarthritis condition characterized by distinct immunological, clinical, genetic, and pathological characteristics, as well as remissions and exacerbations. This study was designed to estimate some Haematological and immunological parameters of Iraqi patients with RA. The data were collected between August 2023 and May 2024 from a total of 60 patients and 30 healthy controls. A serological test was assessed, including erythrocyte precipitation rate, and CRP. Complete blood count was measured using a fully automated quantitative Samsung device. The levels of IL-1β, IL-2, IL-4, IL-10 cytokines were assessed by sandwich ELISA. The result of this study showed that most RA patients were female compared to males. The levels of RF, CRP, and ESR test were significantly higher in patients with RA compared to the control group. RA patients showed statistically significant higher in WBC and Ptt tests while they had lower RBCs, Hb, and RDW compared to controls. When compared to healthy individuals, we observed that patients with RF had greater levels of cytokines IL-1β, IL-2, IL-4, and IL-10. RF patients had higher levels of IL-1β, IL-2, IL-4, and IL-10 than did healthy people.

	2021	AIP
Colorectal cancer is one of most common cancers that cause death, it consider third cause for death. Among the gastrointestinal tract cancers, it is more common in Europe and United States of America than Affrica and Asia. The aim of current study to investigate the association between any molecular aberration in Tp53 gene, exon 5 and mutant p53 protein expression in colorectal carcinoma development. Methods. The current study had two lines: molecular studies in the biology department of the Faculty of Sciences / University of Kufa, and immunohistochemical studies in the histopathology department of the Faculty of Medicine / University of Kufa. The current study was conducted between December 2019 and March 2021. The Scientific Ethical Committee's consents were obtained from all participants' prior to the collection of samples.

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